Validation of a multiplex-tandem RT-PCR for the detection of bovine respiratory disease complex using Scottish bovine lung samples

M. Fergusson, M. Maley, T. Geraghty, J. Palarea Albaladejo, C. Mason, M. S. Rocchi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

The welfare and economic impact of bovine respiratory disease complex (BRDC), and its associated antibiotic usage, are major challenges to cattle rearing and beef cattle finishing industries. Accurate pathogen diagnosis is important to undertake appropriate treatment and long-term management strategies, such as vaccine selection. Conventional diagnostic approaches have several limitations including high costs, long turnaround times and difficulty in test interpretation, which could delay treatment decisions and lead to unnecessary animal losses. We describe the validation of a multiplex-tandem (MT) reverse transcription-polymerase chain reaction (RT-PCR) for the detection of seven common pathogens associated with BRDC. This test has the potential to advance pathogen identification and to overcome many of the limitations of current testing methods. It requires a single sample and results are obtained quickly and not influenced by prior antimicrobial therapy or overgrowth of contaminating organisms. We demonstrated a test specificity of 100% and sensitivity ranging from 93.5% to 100% for these seven common pathogens. This test will be a useful addition to advance BRDC investigation and diagnosis.

Original languageEnglish
Article number106058
JournalVeterinary Journal
Volume303
Early online date14 Dec 2023
DOIs
Publication statusPrint publication - Feb 2024

Bibliographical note

Publisher Copyright:
© 2023 The Authors

Keywords

  • Bovine respiratory disease complex
  • Molecular diagnostics
  • Validation

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